Lund Protein Production Platform (LP3)
Description
Lund Protein Production Platform (LP3) is a Lund University research infrastructure that offers services and equipment in the areas of protein production, crystallization, biophysical characterization, and structure determination. LP3 serves as the Lund University node of the national research infrastructure Protein Production Sweden (PPS, www.pps.gu.se/en) since 2022 and collaborates closely with the MAX IV laboratory X-ray-aided fragment screening platform (FragMAX, https://www.maxiv.lu.se/beamlines-accelerators/science-initiatives/fragmax-biomax-fragment-screening-platform/). For more details about LP3 and what we can do for you, please see its homepage: www.lp3.lu.se/
Offer / Action
LP3 offers services for the entire process chain of production, purification, characterisation, protein crystallisation and protein structure determination and structure refinement or each individual step in the chain. LP3 can help with:
- Plasmids for protein production
- Recombinant protein production in bacterial (E. coli) or eukaryotic (insect) cells.
- Protein labeling (e.g. labeling with stable isotopes (2H, 13C, 15N), biotinylation)
- Protein purification
- Protein crystallisation
- Biophysical protein characterization by multidetector size exclusion chromatography (Omnisec), microscale thermopheresis (MST) & spectral shift (SpS), Dynamic Light Scattering (DLS) and Differential Scanning Fluorimetry (DSF)
- Protein structure determination and refinement
- Application for beamtime. LP3 is part of a Block Allocation Group (BAG) for BioMAX.
- MX data collection at synchrotron beamline (BioMAX MAX IV)
- Process x-ray data and determine and refine the structures
For details of current services and updates, please see the homepage: www.lp3.lu.se
